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KMID : 1007520230320040565
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Food Science and Biotechnology
2023 Volume.32 No. 4 p.565 ~ p.575
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Modulation of gut microbiota by rice starch enzymatically modified using amylosucrase from Deinococcus geothermalis
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Song Eun-Ji
Lee Eun-Sook
So Yun-Sang
Janette Furuzawa-Carballeda
Nam Young-Do
Lee Byung-Hoo
Seo Dong-Ho
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Abstract
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Amylosucrase can increase the amount of resistant starch (RS) in starch by transferring glucose from sucrose to amylopectin. Here, rice starch was modified using amylosucrase from Deinococcus geothermalis (DgAS). DgAS-modified rice starch (DMRS) increased the side-chain length of amylopectin and appeared in the form of B-type crystals. In vitro digestion analyses revealed that DMRS had a higher RS contents and lower digestion rate than native rice starch. When high-fat diet (HFD)-induced C57BL/6 mice were orally administered DMRS, body weight and white fat tissues of DMRS-fed HFD mice were not significantly different. However, serum leptin and glucose levels were significantly decreased and serum glucagon like peptide-1was increased in these mice. The cecal microbiome in DMRS-fed HFD mice was identified to investigate the role of DMRS in gut microbiota regulation. DMRS supplementation increased the relative abundance of Bacteroides, Faecalibaculum, and Ruminococcus in mouse gut microbiota.
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KEYWORD
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Amylosucrase, Rice starch, Resistant starch, Gut microbiota, Deinococcus geothermalis
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